Sample digestion is an important procedure and directly affects on the reliability of quantitative analysis of total selenium in Se-enriched yeast products. The home-digestion closed digestion system was successfully developed by specially designing and constructing from the basic equipments in laboratory that consisted of 4 main devices; water bath, cover plate, vessels and wind tunnel. The optimum conditions of the home-made closed digestion system was accurately and precisely achieved by using 2.0 ml of nitric acid for 0.1 g Se-enriched yeast sample, 100 °C digestion temperature and 20 min digestion time. These conditions showed good validation and acceptation with the certified value of Se-enriched yeast reference material. Furthermore, the digestion efficiency of home-made closed digestion system was as well as the recognized digestion methods especially microwave assisted digestion. Consequently, the home-made closed digestion system could be efficiently utilized as an alternative digestion method for total Se determination in Se-enriched yeast products. Additionally, the home-made closed digestion system was adapted from a basic instrument in laboratory which has affordability and easier operation rather than the recognized methods.
Keywords
Selenium, closed-digestion system, Se-enriched yeast, water bath, wet acid digestion