MAEJO INTERNATIONAL JOURNAL OF SCIENCE & TECHNOLOGY

Volume 15, No. 02, Month MAY, Year 2021, Pages 199 - 208

Apoptotic induction of sub-g1 cells accumulation and cell-cycle-related protein down-regulation in cancer cell lines by hyptis suaveolens (l.) leaf extract

Sumalee Musika, Jirayus Woraratphoka, Korakod Indrapichate

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The effects of Hyptis suaveolens leaf ethanolic extract (HLEE) on cell proliferation of four human cancer cell lines, namely Jurkat, MCF-7, HepG2 and PC-3, were observed. The effects of HLEE on apoptotic cells and cell cycle distribution were analysed and the expression of cell cycle regulatory proteins was assessed. HLEE shows an antiproliferative effect against human cancer cells with a more pronounced activity in Jurkat cells than MCF-7, HepG2 and PC-3 cells. In addition, HLEE also induces cell death in early and late apoptosis, where the dead cells accumulate in the Sub-G1 phase. In addition, HLEE decreases the expression of cyclin D1, cyclin E, CDK2 and CDK4 in a time-dependent manner. The results indicate that HLEE possesses anti-cancer activity in Jurkat cells, induces cell accumulation in Sub-G1, and down-regulates cyclin D1/CDK4 and cyclin E/CDK2 complexes. Thus, H. suaveolens could be a promising phytotherapeutic inhibitor of T lymphocyte leukemia.

Keywords

Hyptis suaveolens, apoptosis, cell cycle, cancer cells, Jurkat cell

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