MAEJO INTERNATIONAL JOURNAL OF SCIENCE & TECHNOLOGY

Volume 15, No. 02, Month MAY, Year 2021, Pages 111 - 128

Identification of mirnas responsive to drought stress in herbaceous peony by high-throughput sequencing

Qi Wang, Yijia Yin, Yongming Fan, Yanyi Zheng, Jaime A. Teixeira da Silva, Xiaonan Yu

Abstract Download PDF

To clarify drought-stress-induced molecular changes in herbaceous peony (Paeonia lactiflora), we construct small RNA libraries by high-throughput sequencing. A total of 58 known miRNAs and 83 novel miRNAs are identified. Among them, 61 miRNAs are differentially expressed. In addition, differentially expressed miRNAs predict 122 target genes, 68 of which are annotated. When combined with gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis, 35 miRNAs and 67 target transcripts that might be associated with drought stress are detected. Expression analysis of some miRNAs are performed, which is consistent with the results of targets of transcription factors and functional genes. These results provide a better understanding of the roles of miRNAs under drought stress in herbaceous peony.

Keywords

herbaceous peony, Paeonia lactiflora, miRNA, drought stress, quantitative real-time polymerase chain reaction (qRT-PCR)

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