Volume 33, No. 02, Month APRIL, Year 2023, Pages -

The production of bacterial cellulose from sweet fermented broken black glutinous rice

Jatupat Samappito, Piyawan Boonchalad, Orawan Tabthaisong

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This research aimed to use sweet fermented broken black glutinous rice (Khao-Mak) as a substrate for bacterial cellulose (BC) production by Acetobacter xylinum. The optimum fermented conditions included Khao-Mak solution preparation, total soluble solid, pH, ammonium sulfate, and starter culture concentration for the production of bacterial cellulose from A. xylinum. The results showed that the optimum condition for the fermentation by A. xylinum in Khao-Mak solution were the ratio of Khao-Mak solution to distilled water at 1 : 2, the total soluble solid content of 10 °Brix, pH 4.0, 0.3% (w/v) ammonium sulphate, and 5% (v/v) of A. xylinum starter. The maximum weight and thickness of bacterial cellulose produced at the optimum condition were 90.15 ± 1.17 g and 14.87 ± 2.26 mm. respectively. When compared texture attribute, bacterial cellulose from Khao-Mak solution had significantly lower score (p < 0.05) of chewiness than that produced from coconut water. However, the color attribute was not significantly different in the bacterial cellulose of both raw materials. Sensory evaluation of BC from Khao-Mak solution and coconut juice was performed by a panel of 30 untrained sensory panels. It was found that there were no significant differences in appearance, color, odor, flavor, texture, and overall preferences. These results revealed that the sweet fermented broken black glutinous rice could be considered as one of the raw materials for the production of bacterial cellulose and an alternative for value added of local raw materials.


Broken Rice; Black Glutinous Rice; Sweet Fermented Rice; Bacterial Cellulose; Acetobacter xylinum


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