This research aimed to study the appropriate parameters for inhibiting microbial growth in Litopenaeus vannamei by plasma-activated water (PAW) by plasma technology to generate high concentration hydroxyl radical (OH•) in hydrogen peroxide (H2O2). The examined factors consist of premix argon and oxygen gas (Ar 98% and O2 2%) flow rate and exposure time. A two-level full factorial design along with five center points was used to determine all experimental conditions. The experiment was performed in order to investigate the microbial growth inhibition phase. Based on the experiment, the optimum conditions were as follows: premix gas flows rate of 25 l/min and exposure time of 120 min., resulting in of H2O2 concentration of 12.43 mg/l. Given 10-min treatment time, plasma-activated water was found to reduce overall microorganisms by 41.63%, from 5.98 logs CFU/g to 3.49 log CFU/g
Keywords
Plasma Activated Water; Vannamei White Shrimp; Inhibition of Microorganism; Hydrogen Peroxide
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