The transplanting of healthy oil palm seedlings from a nursery to the field results in seedlings adaptation to the environment and a high yield produce. This study aims to investigate the effects of biofertilizers viz. arbuscular mycorrhiza (M), phosphate solubilizing fungi (P), and Azolla (A), on growth performance and nutrient content in leaves of oil palm seedlings in mainnursery.Theeight treatments withthreereplications inrandomizedcomplete blockdesign were1)control,2) M,3) P,4) A,5) MP,6) AM,7) AP and8) AMP.The experiment wasconductedat Suratthani Oil Palm Research Center. Growthandnutrientcontent inleaves of oil palm seedlings were analyzed at nineteen months old. The results showed that the growth of seedling, as shown by total dry weight, root dry weight, leave dry weight, and leaf area, under AM application displayed significantly higher than the other treatments. Total dry weight of AM was higher than M and A by 51.52% and 28.75%, respectively. These results corresponded with nutrient content in leaves of AM which was higher than the others. The production cost was increased by 1.7 bath/seedling for AM application.