INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 18, No. 01, Month JANUARY, Year 2022, Pages 421 - 436

Biological activity of rhizobacteria isolated from rhizosphere acacia crassicarpa a. cunn ex benth. in timber plantations

Zul, D., Mustava, M., Diva, A. R., Siregar, B. A., Gafur, A. and Tjahjono, B.

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Acacia crassicarpa is widely planted in timber plantations for pulp and paper industry. However, A. crassicarpa is relatively susceptible to diseases. To control diseases and promote the growth of A. crassicarpa, one of the efforts is utilized rhizobacteria or is known as plant growth-promoting rhizobacteria (PGPR). The biological activity of 27 isolates from the rhizosphere of A. crassicarpa was explored the ability of isolates to produce siderophores, hydrogen cyanide (HCN), indole acetic acid (IAA), and antagonism assays. The ability of isolates to produce siderophores and HCN was determined qualitatively by inoculation of the isolates on iron-free chrome azurol S agar and King’s B medium, respectively. IAA production was estimated colorimetrically by culturing the isolate on nutrient broth containing Ltryptophan as a precursor. The characteristic of the isolates capable of producing IAA is the color change of the culture after the addition of Salkowski’s reagent. Antagonisms assay on pathogens that cause leaf blight and wilt were performed by using a cross-streaking method and dual culture method, respectively. ETC...

Keywords

Acacia, HCN, IAA, Rhizobacteria, Siderophores

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