ThaiScience  


ENIVRONMENT ASIA


Volume 14, No. 03, Month SEPTEMBER, Year 2021, Pages 117 - 123


Evaluation of potential genotoxic effects induced by chlorothalonil in human lymphocytes in vitro

Awad A Algarni


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Chlorothalonil is a widespread non-systemic fungicide used for the control of fungal infections in agriculture of vegetables and fruits. Due to the widespread use of chlorothalonil, chlorothalonil has been detected in all environmental systems, which poses potential risks to human health. The genotoxic eff ects of the fungicide Chlorothalonil were analyzed in cultured human peripheral lymphocytes by use of cytogenetic parameters including chromosomal aberrations, sister chromatid exchange and mitotic index. Blood samples were obtained from two healthy Non-smoker volunteers (aged 25 to 26 year old). Human lymphocytes were treated with four diff erent concentrations of Chlorothalonil (1, 5, 20, and 50 µg/mL). The results of this study showed that Chlorothalonil signifi cantly increased the frequency of chromosomal aberrations and sister chromatid exchange compared to the negative control. Moreover, chlorothalonil signifi cantly decreased the mitotic value at all concentrations. Current fi ndings show that Chlorothalonil is clastogenic and induces in vitro DNA damage in human lymphocytes.


Keywords

Chlorothalonil; Genotoxicity; Chromosome Aberrations; Mitotic Index; sister chromatid exchange; human peripheral lymphocytes



ENIVRONMENT ASIA


Published by : Thai Society of Higher Education Institutes on Environment
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