SRINAGARIND MEDICAL JOURNAL

Volume 35, No. 03, Month MAY, Year 2020, Pages 261 - 265

Effectiveness screening of hemoglobin e using kku-dcip in hiv infected patients

Nattaphol Prakobkaew, Kanokporn Rittisang, Sitthichai Panyasai, Surachat Buddhisa, Orrathai Pongtussanaham, Narin Pongtussanaham

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Background and Objective: Human immunodeficiency virus (HIV) is a cause of acquired immunodeficiency syndrome. Hematological abnormalities are common manifestations in persons with HIV infection. HIV infection can occur during pregnancy and may be affected on the screening for hemoglobin E (Hb E) by false positives or false negatives. In this study, we aimed to investigate the effectiveness of Hb E screening using KKU-DCIP test in HIV positive subjects. Methods: Two hundred subjects of HIV infected patients and healthy donors were enrolled to this study. Hb E screening using KKU-DCIP test and allele specific-PCR for Hb E gene confirmation were performed in all samples. Results: The result in HIV infected patients showed that the false positive, sensitivity, specificity, positive predictive value and negative predictive value using KKU-DCIP test were 22.58%, 100%, 96.0%, 77.4%, and 100%, respectively. In non-HIV infected group, false positive rate of Hb E screening using KKU-DCIP test was 28.13. All of sample, false negative was not detected. Sensitivity, specificity, positive predictive value and negative predictive value were calculated, the results were 100%, 94.9%, 71.9% and 100%, respectively. Conclusion: The results indicate that KKU-DCIP test still has effectiveness for screening of Hb E in HIV infected patients.

Keywords

Hemoglobin E, HIV infected, KKU-DCIP test

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