SRINAGARIND MEDICAL JOURNAL

Volume 35, No. 04, Month JULY, Year 2020, Pages 418 - 424

Comparative study of characterization of mesenchymal stem cells from amnion and chorion of fetal membrane

Sasiprapa Thongbopit, Suparat Wichitwiengrat, Puttachart Pokathikorn, Tatsanee Phermthai

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Background and Objective: The fetal membranes, amnion and chorion, are the attractive sources of mesenchymal stem cells (MSCs). However, the comparative study between the amnion and chorionderived MSCs are still unclear. This study aimed to isolate and compare the properties of the amniotic mesenchymal stem cell (AMSC) and chorionic mesenchymal stem cell (CMSC). Methods: AMSC and CMSC were isolated from the fetal membranes (n=5). The morphologies, population doubling times (PDT), MSC surface markers, and multilineage differentiation potentials of AMSC and CMSC were compared. Moreover, the viability of both MSCs were investigated after cryopreservation. Results: AMSC and CMSC exhibited similar morphologies. Both expressed CD44, CD73, CD105 but not CD34 on the cell surfaces and could be differentiated into adipogenic, osteogenic, and hepatogenic cells. As for PDT, AMSC proliferated faster than CMSC, but this was not reached statistically significant. After the cryopreservation for 1 month, more than 90% of AMSC and CMSC were viable. Conclusions: The results of this study demonstrated that AMSC and CMSC had similar characteristics and differentiation potential. Therefore, both amnion and chorion are the alternative sources of MSCs, which might be useful for therapeutic applications.

Keywords

fetal membrane mesenchymal stem cells amnion chorion

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