The objectives of this research were to develop of transmission line calculation program based on usage and frequency consisting of the two-wire transmission line, twisted pair transmission line, and coaxial transmission line. The program was developed in the form of graphical user interface in Matlab. The developing and testing results found that the program was correctly capable to calculate capacity, conductance, inductance, resistance and impedance for each transmission line usage and frequency. The results from the program with black box testing by five experts and twenty system users found that the average and the standard deviation of results form computer experts were 4.04 and 0.36, respectively, while those of results form the system users were 3.88 and 0.48, respectively. Conclusion, it showed that the efficiency of the calculation program was a high level and can be used as a teaching and learning as well.