SILPAKORN UNIVERSITY SCIENCE AND TECHNOLOGY JOURNAL

Volume 12, No. 01, Month JANUARY, Year 2018, Pages 19 - 32

Anti-inflammatory effects of rhein and crude extracts from cassia alata l. in hacat cells

Nushjira Pongnimitprasert, Kwanchanok Wadkhien, Chatchai Chinpaisal, Malai Satiraphan, Penpan Wetwitayaklung

Abstract Download PDF

Cassia alata L. is a medicinal plant and is in the Thai traditional household herbal drug list. Furthermore, C. alata leaves extract has been reported to have anti-inflammatory activities. The rhein, an active component, can inhibit inflammation via suppressing reactive oxygen species (ROS) production, leading to decreased cytokines (TNF-α, IL-8). However, the role of anti-inflammation on skin has been investigated only in a few research. The rhein in C. alata leaves extract was determined by HPLC. The anti-inflammatory effects of rhein and C. alata leaves extract on tert-butyl hydroperoxide-induced oxidative stress in HaCaT cells were evaluated. Anti-inflammatory activities of C. alata leaves extract were compared with rhein standard via inhibition of ROS generation and production of TNF-α and IL-8. The results showed that rhein content in C. alata leaves extract was 0.1225% w/w. Rhein (1-50 μM) significantly reduced ROS generation in a concentration-dependent manner, and paralleled the decrease in TNF-α and IL-8 production. C. alata leaves extract exhibited stronger anti-inflammatory effect than rhein at same concentration. These findings indicate that rhein and C. alata leaves extract may reduce inflammation by decreasing TNF-α and IL-8 as a result of ROS reduction. These results indicate that C. alata leaves may have the possibility of role as an anti-inflammatory agent. Therefore, further studies need to be performed.

Keywords

Cassia alata, rhein, HaCaT cell, ROS, anti-inflammatory

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