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THAMMASAT INTERNATIONAL JOURNAL OF SCIENCE & TECHNOLOGY


Volume 25, No. 02, Month APRIL, Year 2020, Pages 86 - 96


Accelerated stability study on anti-allergic, anti-inflammatory activities and phytochemical contents of the ethanolic extract of zingiber officinale roscoe

Rodsarin Yamprasert, Waipoj Chanvimalueng, Napaporn Pattanacharoenchai, Weerachai Pipatrattanaseree, Nichamon Mukkasombut, Arunporn Itharat


Abstract Download PDF

Allergic rhinitis (AR) is a global health problem and herbs are one of the alternative treatments for AR. Ginger is widely used as a spice food. Many reports have revealed that ginger extract has potential anti-allergic and anti-inflammatory activities. However, there is no report on the stability of the anti-allergic and anti-inflammatory activities of ginger extract after storage. This study aimed to investigate the stability of ginger extract on the accelerated condition by determining anti-allergic and anti-inflammatory activities and measuring 6-gingerol and 6-shogaol. The ethanolic extract of ginger was kept under the accelerated condition for six months. The extracts at various storage times were investigated for antiallergic and anti-inflammatory activities by measuring the inhibition of β-hexosaminidase release from RBL-2H3 cells and nitric oxide production in RAW 264.7 cells, respectively. Levels of 6-gingerol and 6-shogaol were determined by HPLC. The results showed that under the accelerated storage condition, the anti-allergic activity of ethanolic ginger extract was stable until day 90; however, after day 90 the IC50 values were still less than 20 μg/ml. There was no significant difference in anti-inflammatory activity of all stored ginger extracts comparing to day 0. At the end of the stability storage, the level of 6-gingerol, one of major components, was reduced, but 6-shogaol level was increased. However, the anti-allergic and anti-inflammatory activities were maintained at acceptable levels. Therefore, ginger extract can be stored within 2 years at room temperature without the loss of both activities. This study provided the stability data for development of ginger extract to be a drug for AR treatment.


Keywords

Ginger, Anti-allergic activity, Anti-inflammatory activity, HPLC, Stability study



THAMMASAT INTERNATIONAL JOURNAL OF SCIENCE & TECHNOLOGY


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