SCIENCE ASIA

Volume 46, No. 02, Month AUGUST, Year 2020, Pages 392 - 396

A phospholipase a2 of daboia siamensis venom suppressed expression of genes and proteins in mapk pathway

Suchitra Khunsap, Orawan Khow, Sunutcha Suntrarachun, Supatsorn Boonchang

Abstract Download PDF

High mortality rate of Melanoma is due to poor response to radiotherapy and chemotherapy. A phospholipase A2 (PLA2) of Daboia siamensis venom has been reported to inhibit melanoma cells. The aim of this study is to evaluate the effects of dssPLA2 on gene and protein expression of MAPKs (Mitrogen-activated protien kinases) in melanoma cells. The mRNA and protein were determined by quantitative real-time PCR and western blotting to detect the expression levels of MEK1, MEK2, BRAF V600E and ERK1/2. Results indicated that 0.25 µg/ml of dssPLA2 reduced the mRNA expression level of these genes in a time dependent manner. Interestingly, at 48 h, the mRNA expression level of all genes was extremely decreased compared with untreated cells in the same condition. Whereas, the protein level of MEK1, MEK2, BRAF V600E and ERK1/2 were clearly reduced after dssPLA2 incubation. Our results show that dssPLA2 is a strong inhibitor to MAPK genes at 48 h.

Keywords

melanoma, MAPK pathway, phospholipase A2, Daboia siamensis venom, BRAF V600E

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