Aflatoxin B1 (AFB1) is one of mycotoxins leading to serious food contaminations and human diseases.
To solve these problems, biodegrading technique has been concerned. Previous researches showed that AFB1 can be
effectively degraded by the culture supernatant of many microorganisms. However, the biodegraded products have
not been identified. In this study, to explore the structure of the products, the AFB1 was degraded by the culture
supernatant of Cladosporium uredinicola. Two biodegraded products of AFB1 were collected by high performance
liquid chromatography (HPLC) and analyzed by liquid chromatography quadruple time-of-flight mass spectrometry
(LC-Q-TOF MS) and chromatography-tandem mass spectrometry (MS/MS). As a result, the molecular weight of the
two products were 406 and 342, and the structure of the two products were inferred. The toxicity of the biodegraded
products was evaluated based on quantitative structure-activity relationship (QSAR) and cytotoxicity experiment. In
conclusion, the double bonds of AFB1 had a high probability of being destroyed. The toxicity of the two biodegraded
products compared with that of AFB1 was reduced. The culture supernatant of C. uredinicola was used as an effective
way to detoxify AFB1.