SCIENCE ASIAVolume 46, No. 02, Month FEBRUARY, Year 2020, Pages 133 - 141
Low expression of microrna 203a in mid-gestational human fetal keratinocytes contributes to cutaneous scarless wound healing by targeting tenascin c
Meng Chena, Feng Zhaoa, Zhe Wangb, Hongxin Langa, Kun Zhanga, Tao Zhanga, Rui Wanga, Ping Shic, Xining Panga,c,*
Abstract Download PDFThis study aimed to detect the roles of miRNA-203a in fetal cutaneous regeneration. The levels of miR-203a in eight mid-gestational and ten late-gestational fetal epidermis tissues were detected by real-time qRT-PCR. The expressions of α-SMA, TGF-β2 and TGF-β3 were detected by real-time qRT-PCR and Western blot. Moreover, the proliferation and migration abilities of keratinocytes (KCs) and fibroblasts (FBs) were examined by using MTS and transwell cell migration assays. Bioinformatics analyses, luciferase reporter and ELISA assays were used to examine the potential target genes. The results showed that miR-203a was dramatically up-regulated in late-gestational fetal epidermis tissues. MiR-203a inhibited the migration and proliferation abilities of KCs and FBs. Tenascin-C (TNC) was a direct target of miR-203a and miR-203a regulated TGF-β isoforms and α-SMA. It was concluded that the low-expression of miRNA-203a promoted KCs and FBs migration by targeting TNC and regulating TGF-β isoforms and α-SMA.
miR-203a, fetal skin, wound healing, fibrosis, TNC