SCIENCE ASIAVolume 46, No. 02, Month FEBRUARY, Year 2020, Pages 128 - 132
Phylogenetic diversity of cultured bacteria from prevalent species of corals around samae san island, thailand
Dewi Embong Bulan, Suchana Chavanich, Voranop Viyakarn, Naraporn Somboonna
Abstract Download PDFPredominant corals of Samae San island, Thailand, including Acropora humilis, Acropora millepora, Porites
lutea and Platygyra sinensis, were cultured and identified for bacterial species by 16S rRNA gene sequencing. Of all
corals, dominant cultured bacteria were Firmicutes (46.75%), Proteobacteria (34.60%), Actinobacteria (17.18%) and
Bacteriodetes (1.47%). Firmicutes such as Staphylococcus, Bacillus and Sediminibacillus was relatively most abundant
(50%), except in P. sinensis that Proteobacteria was more abundant. Over culture temperature range of 20–50 °C,
different bacterial species were grown (ANOVA, p < 0.05). Coral P. lutea and A. humilis associated bacteria were able
to be cultured at the highest temperature (45 °C), followed by coral A. millepora (40 °C) and P. sinensis (35 °C) bacteria.
The high-temperature cultured bacteria were mostly Bacillus such as Bacillus amyloliquefaceins. Multiple sequence
alignment and phylogeny relationship of the bacterial species from these four corals showed that, for Firmicutes and
Proteobacteria, the bacterial species isolated from coral P. lutea, A. humilis and A. millepora rather shared clades. Overall,
the coral Acropora demonstrated more diversity of bacterial species than coral Porites. The culturing attempt at high
temperature allowed additional bacterial species findings.
bacterial diversity, 16S rRNA gene sequencing, coral-associated bacteria, Thailand