The conservation of teak (Tectona grandis L.) by slow growth was proceeded out
to determine appropriate methods in vitro preservation using osmotically active compounds
and growth retardants. Calli and node explants were cultured on Murashige and Skoog (MS)
and Woody Plant Medium (WPM) media supplemented with 1, 2 and 3 mg· L-1 abscisic acid
(ABA) containing 30 g·L-1 sucrose and hormone-free MS and WPM media containing 0.1,
0.2 and 0.3 M sorbitol for reduced the growth rate. Result showed that preservation of calli
on MS and WPM media supplemented with 0.2 and 0.3 M sorbitol and node explants were
cultured on WPM medium supplemented with 0.2 and 0.3 M sorbitol inhibited the growth
and maintained the quality of calli and node explants for 12 weeks. It was found that ABA
could not inhibit the growth rate after cultures for 12 weeks. Callus and node explant survival
from slow growth preservation were able to regrowth which successfully (100%) tested on WPM
medium supplemented with 0.25 mg·L-1 6-benzylaminopurine (BAP) containing 30 g·L-1
sucrose after for 2 weeks.
Keywords
Growth retardants, Mahesak (Teak), Preservation, Slow growth, Tectona grandis L.
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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