INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 16, No. 05, Month SEPTEMBER, Year 2020, Pages 1199 - 1216

Effect of nitrogen fertilizer and azospirillum product on growth of rice variety pathum thani 1 and bacterial diversity in the rhizosphere

Preepremmot, P., Amkha, S., Chungopast, S. and Mala, T.

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The application of Azospirillum product (A), A with a half dose or three-quarter dose of nitrogen fertilizer produced greater nitrogenase activity (533–634 nmol C2H4/plant/h) than the control. Moreover, these treatments produced a grain yield (6,001.3–6,480.6 kg/ha) as high as using 100% nitrogen fertilizer. The bacterial community richness and diversity in the rhizosphere soil were higher than for the rice roots after metagenomic analysis based on 16S rDNA sequencing. Proteobacteria was the major phylum in all samples. The numbers of Azospirillum spp. in both the rhizosphere soil and rice roots treated with A and A supplemented with a half dose of nitrogen fertilizer were higher than for other genera. The results indicated that the application of A promoted not only nitrogenase activity, straw yield, and rice yield but also bacterial community diversity.

Keywords

Azospirillum, Rice, Bacterial community diversity, Bacterial community richness

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