INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 16, No. 05, Month SEPTEMBER, Year 2020, Pages 1175 - 1184

In vitro propagation from nodal segments of arachis glabrata cultivar florigraze

Pholjad, A., Pongtongkam, P., Arananant, J. and Poeaim, A.

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Arachis glabrata Benth. of rhizome perennial peanut is used as a forage plants with higher nutrient value. The nodal segments were used to propagate in vitro by 0.2% of HgCl2 plus antibiotic and cefotaxime and preservative for plant tissue culture media active (PPM) at 15 min. It was the most effective procedure for disinfecting plant parts (survival of 73.33%). Shoot induction used to the nodal segments of A. glabrata cultivar Florigraze were cultured on MS medium combination with plant growth regulators in cytokinin group at different concentrations 0.5, 0.75, 1, 2, 3 and 5 mg L-1 of 6-benzylaminopurine (BAP), N6- furfuryladenine (Kn), meta-Topolin (mT) and Thidiazuron (TDZ). The maximum growth rate was 66.67% on 5 mg L-1 of mT was the highest shoot length of 18.43 mm after 30 days. Root induction was achieved on MS medium supplemented with 2 mg L-1 of α-Naphthaleneacetic acid (NAA) and 0.2% Activated Charcoal (AC) after 8-12 days cultured when compared with MS medium without plant growth regulators at the basal condition. Thereafter, the plantlets were transferred into the soil for acclimatization. It is the first report using the nodal segment in vitro propagation.

Keywords

Activated Charcoal, Arachis glabrata cultivar Florigraze, Nodal segment, Shoot induction

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