INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 16, No. 05, Month SEPTEMBER, Year 2020, Pages 1125 - 1144

Identification of biostimulant and microbicide compounds from streptomyces sp. uc1a-3 for plant growth promotion and disease control

Manigundan, K., Joseph, J., Ayswarya, S., Vignesh, A., Vijayalakshmi, G., Soytong, K., Gopikrishnan, V. and Radhakrishnan, M.

Abstract Download PDF

The plant growth promotion and antagonistic potential of Streptomyces against phytopathogens was assessed. Total fourteen Streptomyces strains were derived from rhizosphere soil of Capsicum annum (Chilli) from the agricultural fields in Udhagamandalam, Nilgiris, Tamil Nadu, India. All strains were evaluated for plant growth promoting in vitro e.g. production of indole acetic acid, ammonia, siderophores, chitinase, cellulase, protease, amylase and inorganic phosphate solubilisation. In addition, antagonistic activity was also tested against Ralstonia solanacearum, Xandhomonas oryzae, Fusarium oxysporum, Alternaria sp., Macrophomina sp., and Magnaporthe oryzae. Further, bioactive compounds from the strain UC1A-3 was analyzed through gas chromatography–mass spectrometric technique. Three strains showed the highest level to promote plant growth promoting and antagonistic activity especially the strain UC1A-3 revealed maximum level of seed germination and increased shoot and root length in Chilli plants. Totally, twenty-nine compounds were detected, most of which were aromatic compound derivatives. In particular, Phthalic acid (C8H6O4), Pentadecanoic acid (C15H30O2), i-Propyl 12-methyltetradecanoate (C18H36O2), l-(+)-Ascorbic acid 2,6- dihexadecanoate (C38H68O8), 1-Nonadecene (C19H38), 1-Heptacosanol (C27H56O) were reported as antimicrobial properties. Findings of the present study evidenced that Streptomyces strain UC1A-3 would be a promising candidate for agricultural crop improvement, since it has showed the potential in-vitro plant growth and biocontrol activities against the tested phytopathogens.

Keywords

Streptomyces; Bioactive metabolites; Plant growth promotion; Gas chromatography–mass spectrometry; Phthalic acid

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