A protocol of micropropagation is established for Ficus carica L. cv. Black Jack
with excised apical buds from one-year-old mother plant as in vitro explant materials. The
response of explants on BAP and 2-iP at different concentrations (0, 10, 20, 30 and 40 μM)
were investigated via histological analyses and scanning electron microscopy (SEM).
Isodiametric cells and vascular tissues were observed from the cross-sections of apical buds
under the treatments of BAP and 2-iP at all concentrations via SEM. Histological analysis
confirmed that both cytokinins tested at different concentrations involved the formation of
actively dividing meristematic cells prior to differentiate to parenchymatous tissues. It is
also proven that more vascular tissues were found at lower epidermal layers. Histological
analysis was proved that better response for callus induction was obtained by the adaxial
surface on the leaf in contact with the media. SEM analysis confirmed that no structural
distortion caused by cytokinins.
Keywords
Fig , BAP, 2-iP, Microscopy analysis
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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