SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 43, No. 01, Month JANUARY, Year 2021, Pages 169 - 180

Light-sheet microscopy for high-resolution imaging of caudoeuraphia caudata (pilsbry, 1916), a new record of acorn barnacle from thailand’s coast and its application in taxonomic identification

Woranop Sukparangsi, Chinnakit Wongkunanusorn, Pisit Sanjan, Sutin Kingtong, Salinee Khachonpisitsak

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The acorn barnacle (Cirripedia: Balanomorpha) is a sessile crustacean arthropod, distributing around the intertidal areas of tropical and temperate regions worldwide. Current practices for taxonomic identification are based on shell morphology and light microscopy, together with the use of scanning electron microscopy for arthropodal characters, which the latter technique requires complicated procedures. Through the recent technology of confocal light-sheet microscopy, here we demonstrate a clear description of Caudoeuraphia caudata (Pilsbry, 1916), a new record of its presence in eastern Thailand. This type of microscopy enables the high acquisition of fluorescent imaging of a whole barnacle’s body and arthropodal structures, including cirri and mouthpart imaging in three dimensions, with simple procedures for sample preparation and through harboring autofluorescence of their own barnacle structures. Hence, this technology could potentially be an alternative way for identifying acorn barnacles at the species-level and visualizing the diversity of these marine arthropods.

Keywords

light-sheet microscopy, barnacle, Cirripedia, Chthamalidae, Caudoeuraphia caudata

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