SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 42, No. 05, Month SEPTEMBER, Year 2020, Pages 975 - 983

Torvoside a extracted from solanum torvum fruits does not directly affect 3t3-l1 adipogenesis, adiponectin synthesis and glucose uptake

Pilaiwan Siripurkpong and Mudtika Fungkrajai

Abstract Download PDF

The present study was aimed to determine the effects of Torvoside A from methanolic extract of Solanum torvum on adipogenesis, fat accumulation, and adiponectin synthesis as well as glucose uptake activities. For adipogenesis assay, 3T3-L1- preadipocytes were treated with Torvoside A (0, 5, 25, 50 μM) during 7 days of adipocyte differentiation, while 3T3-L1 mature adipocytes were treated with Torvoside A for 2 days in the fat accumulation study. There were no significant differences in the intracellular triglyceride among Torvoside A treated cells and the untreated control (p>0.05) in both assays. Torvoside A did not significantly change adiponectin synthesis in both transcriptional and translational levels (p>0.05), measured by real-time RTPCR and western blot, respectively. Finally, Torvoside A did not affect glucose uptake activity and GLUT4 transcript as well. These results can be concluded that Torvoside A could not directly promote adipocyte hyperplasia and hypertrophy as well as the anti-diabetic activities.

Keywords

Torvoside A, adipogenesis, glucose uptake, Solanum torvum, fat accumulation

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