RNA interference mediated-silencing of the F3H gene in the lotus (Nelumbo nucifera
Geartn.) cv. Buntharik inhibited the anthocyanin pathway after gene transformation. The plasmid
pJA8F3H contained the inverted repeats of F3H fragments, bar gene as a selectable marker in
plants, which is resistant to basta (glufosinate amonium). The transformation of particle
bombardment was obtained with multiple shoots of lotus. F3H gene expression analysis showed
that expression of F3H decreased in transgenic lotus when compared with the control. On the
first and the second test, 5 transgenic lotus of the relative expression F3H gene was detected
with 0.0496 and 0.466 times, respectively. The inhibition of gene expression decreased when
they were cultured for a long time. The expression F3H gene was detected by RT-PCR, and
real-time PCR resulted in consistent decreasing in the transgenic lotus compared with the nontransgenic
lotus, which was able to inhibit the expression F3H gene.
Keywords
Nelumbo, Particle bombardment, Bar gene
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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