The potential probiotic lactic acid bacteria (LAB) were isolated from intestinal
contents and gills of Nile tilapia (Oreochromis niloticus). Those properties included
antagonistic activity against pathogenic bacteria, tolerance to pH, NaCl, fresh bile from Nile
tilapia. Moreover, drug resistance profiles were examined. A total of forty isolates were
collected. LAB isolated from the intestinal contents showed inhibitory activity against Bacillus
coagulans TISTR1447, Pseudomonas fluorescens TISTR358 and Salmonella Typhimurium
TISTR292, whereas those from the gills revealed only antagonistic activity against Bacillus
coagulans TISTR1447, S. Typhimurium TISTR292 and Escherichia coli TISTR780. Isolates
LI10 and LG5 were selected for further evaluation of probiotic properties. Both isolates could
grow in pH 4–10, NaCl concentration up to 10% and fresh bile concentration up to 10%. These
isolates were resistant to cefovecin, marbofloxacin, clindamycin and
trimethoprim/sulfamethoxazole, meanwhile they were susceptible to benzylpenicillin,
amoxicillin/clavulanic acid, vancomycin, tetracycline, nitrofurantoin and chloramphenicol. In
addition, both isolates were identified by Matrix-assisted lazer desorption/ionization-time of
flight mass spectrometry (MALDI-TOF MS) as Enterococcus feacalis. Therefore, these two
isolates could potentially be used as probiotics.
Keywords
Probiotics, Nile tilapia, Gills, Intestine, Lactic acid bacteria
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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