Isaria tenuipes is an insect-parasitic fungus, an anamorph of Cordycep takamontana
and used a nutraceutical in traditional Chinese medicine for cancer patients. In vitro cytotoxic
activity of extracts from I. tenuipes VHI-2 on cancer cell. This I. tenuipes strain was collected
in the Langbiang mountain, Tam Dao and Ba Vi national parks from Vietnam and cultured to
collect biomass and fruit bodies on liquid at optimal conditionat 20-220C. We obtained 7
extracts from biomass and 6 extracts from fruit bodied via percolation method with ethanol
(EtOH) and liquid-liquid extraction method by four different solvents: petroleum ether (60 –
80oC) (PE), etyl acetate (EA), butanol (BuOH) and water (W), successively. The extracts were
proved in vitro cytotoxic activity against MCF-7 breast carcinoma cell line by the
sulforhodamine B (SRB) assay at 100 μg/mL concentration for 48 hours. The result showed
that fruit-body extracts were shown higher cytotoxic potential than biomass extracts but all
were below 50%. Specifically, EA extract of fruit body had the highest with inhibited
percentage 44.83 ± 6.88%, the other expressed approximate activities as in PE, BuOH, W, PS
with percentage of inhibition were 9.86 ± 5.44%, 26.98 ± 2.82%, 7.97 ± 6.88% and 3.40 ± 3.06%
respectively. Meanwhile, only PS extract of biomass has active (31.35 ± 6.08%). These results
indicated that I. tenuipes VHI-2 express low ability to inhibit MCF-7 cancer cell line and more
researches are needed on other cancer cell lines to determine correctly potential of I. tenuipes
VHI-2 in cancer treatment.
Keywords
Isaria tenuipes, Cytotocity, MCF-7 cell line
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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