INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
Volume 16, No. 04, Month JULY, Year 2020, Pages 855 - 866
Optimization of culture parameters of selenium-enriched ophiocordyceps sinensis biomass by response surface methodology
Le, Q. P., Nguyen, T. H., Huynh, V. P., Nguyen, T. T. M., Tran, M. T., Dinh, M. H. and Ngo, K. S.
The medium composition and culture conditions of 25 mg/L selenium supplementing
Ophiocordyceps sinensis was optimized by response surface methodology. The results showed
that the optimum medium for Se enrichment of O. sinensis found to be 53.48 g/L saccharose,
11.17 g/L peptone, 1.05 g/L KH2PO4. The biomass and selenium content was 24,93 g/L, 960,31
μg/g, respectively. The obtained optimum conditions for O. sinensis consisted of daylight,
temperature 20 °C, initial pH value 6.1. The maximum biomass and total Se yield reached
26,45 g/L, 1068 μg/g, respectively. The result indicated that response surface methodology is a
promising method for the optimization of selenium-enriched O. sinensis fermentation process,
and which is the basis for further studies of the fungal bioactivity.
Keywords
Biomass, Ophiocordyceps sinensis, Optimization, Selenium, Response surface methodology