Rice blast caused by Magnaporthe oryzae (anamorph: Pyricularia oryzae) is a
destructive disease in all rice growing regions of the Karnataka. The morphological and
biochemical variations existing among the field isolates collected from different agro-climatic
zones was investigated. The influence of culture media on growth, colony character of M.
oryzae isolates on two different media viz., potato dextrose agar and oat meal agar was studied.
Results revealed that there was a significant variation among the isolates of M. oryzae in
different parameters such as growth pattern, colour of media and mycelia, texture and
sporulation (P ≤ 0.05). These variations are supported by isozyme analyses which showed
significant level of variations among the isolates with 17 polymorphic bands. In UPGMA
cluster analysis, the coefficient of similarity ranged from 0.26 to 1. Two distinct clusters were
formed among the isolates i.e., from the southern districts (cluster I) and northern district
isolates (cluster II). This suggested that there is a correlation among the geographical
boundaries with the diverse isozyme profile of the pathogen for its adaptability to the different
environmental conditions.