Availability of cis-1,4-polyisoprene from Hevea brasiliensis has become important for many kinds of products, as it is
used in automobiles, gloves, and adhesives. Increasing the yield of latex from rubber trees would contribute to income in tropical
countries. The latex yield depends on various factors, including plant hormones and aging. This work focused on studying the
effects of these factors on dry rubber content and on proteome profiles. The results suggest that dry rubber content slightly
decreased upon hormonal treatment, and increased with maturation. Both factors affected the protein concentration. Comparative
proteomic analysis indicated that ethylene stimulation altered abundances of 1,553 soluble proteins and 1,001 rubber-bound
proteins. Plant maturation affected abundances of 2,961 soluble proteins and 839 rubber-bound proteins. These results provide
useful information about the protein-level mechanisms of rubber biosynthesis, affecting rubber yield in response to hormone
treatment and aging.