INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
Volume 16, No. 02, Month MARCH, Year 2020, Pages 403 - 420
Effect of extracted pectin from fruit wastes on growth of pediococcus pentosaceus rsu-nh1 and lactobacillus plantarum rsu-so2
Showpanish, K., Sonhom, N., Pilasombut, K., Woraprayote, W., Prachom, N., Buathong, R., Yodsenee, K., Somsri, A. and Rumjuankiat, K.
Two strains of lactic acid bacteria identified as Pediococcus pentosaceus RSU-Nh1
and Lactobacillus plantarum RSU-SO2 were isolated from Thai fermented pork sausage
(Nham) and fermented spring onion (Ton-Hom-Dong) respectively. The culture supernatant
(CFS) of both strains exhibited antimicrobial activity against both Gram-positive and Gramnegative
pathogenic bacteria. After being treated with alpha-chymotrypsin and trypsin, the
antimicrobial activity of their CFS was eliminated, which suggests that the antimicrobial
compound produced was antimicrobial peptide or bacteriocin. Pectin extracted from different
fruit peels was supplemented in the bacterial growth medium to investigate the effect of pectin
on the growth and antimicrobial production of Ped. pentosaceus RSU-Nh1 and Lb. plantarum
RSU-SO2. Pectin was extracted from pomelo, durian, passion fruit and salacca fruit peels with
a pectin yield of 5.02 to 26.44%, degree of esterification of 80.84 to 86.68%, and galacturonic
acid content of 52.36 to 64.23%. Supplementation with pomelo and passion fruit pectin
exhibited the highest improvement of bacterial growth and antimicrobial production of Ped.
pentosaceus RSU-Nh1 and Lb. plantarum RSU-SO2. These findings suggested the potential
application of these pectin extracts as prebiotics to promote the growth and antimicrobial
production of lactic acid bacteria.
Keywords
Pectin, Fruit waste, Lactic acid bacteria, Bacteriocin