The mutation of chrysanthemum with ray florets was induced by colchicine. The
explants were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg/l NAA
and 4 mg/l Kinetin for inducing calli for 12 weeks. The shoots were regenerated from callus
when cultured on MS medium for 8 weeks. The plant growth, size of stomata and number of
chloroplasts per stomata were not statistically different in each treatment. However, the ray
florets were received high levels of colchicine is less to survivor rate. The plantlet of
chrysanthemum was transfered to pot plant containing soil and chopped coconut. Five of ten
plant showed the flowers color changed from purple into a pinkish-orange at the 0.10%
colchicine for 12 hours. The explant obtained from soaked of 0.15 % colchicine for 12 hours
with the highest number of flowers per plant, 72 flowers per plant.