INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 16, No. 01, Month JANUARY, Year 2020, Pages 175 - 188

Morphological and molecular based identification of trichoderma cb-pin-01 biological control agent of plant pathogenic fungi in thailand

Unartngam, J., Srithongkum, B., Intanoo, W., Charoenrak, P. and Chamswarng, C.

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Trichoderma CB-Pin-01 is an efficient biocontrol agent of fungal plant diseases, commercially produced and legally registered in 1996 as a biological control agent against several plant diseases in Thailand. This isolate was primarily identified as T. harzianum based on morphological characteristics. To confirm the species identification of Trichoderma CB-Pin-01, conidial surface observation and DNA sequence analysis were conducted. Trichoderma CB-Pin-01 showed irregular pyramidal warts on conidia surface which is a typical characteristics of T. asperellum. Moreover, ITS 1 and 4 sequences of rDNA combined with tef1 gene sequence indicated the phylogenetic placement of Trichoderma CB-Pin-01 as T. asperellum. We concluded that the formerly T. harzianum CB-Pin-01 has to be re-classified as Trichoderma asperellum.

Keywords

Trichoderma, Trichoderma asperellum, Biological control, Plant disease

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