INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 15, No. 06, Month NOVEMBER, Year 2019, Pages 1021 - 1032

Prevalence and genetic diversity of trypanosoma evansi infection causing abortion among cattles and buffaloes in eastern border area of thailand-cambodia

Srikijkasemwat, K. and Kaewhom, P.

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Trypanosoma evansi, a protozoan blood parasite in animals, causes surra disease and easily leads to abortion in cattle and buffaloes. The results demonstrated that the PCR product was 164 bp in length. The prevalence of T. evansi infection in cattle and buffaloes of Ta Phraya, Khok Sung, Aranyaprathet and Khlong Hat districts in Sa Kaeo province was 19.67% (12/61), 38.57% (27/70), 45.16% (70/155) and 35.45% (25/92), respectively. The satellite DNAs (TBR primer) were analyzed and revealed that it could demonstrate the genetic diversity of T. evansi of cattle and buffaloes. Tree construction based on the satellite DNAs in each district of border areas confirmed the close relationship between cattle and buffalo. The results found that trypanosome minor variations might be due to livestock system, a pasture or forest grazing. These feeding were difficult to get rid of insects that are disease vectors such as tabanidae, flies, and mosquitoes as well as easy to spread or transmission of trypanosome.

Keywords

Trypanosoma evansi, Cattle, Buffalo, Eastern border area of Thailand-Cambodia

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