Artemisia annua is a medicinal plant for antimalaria that has been used for hundred
years ago. Many techniques have been applied to increase production of artemisinin including
irradiation of Gamma rays in order to obtain mutant plants. Mutation is also attempted to
increase genetic variability. The cluster of shoots culture of A. annua after Gamma irradiation
treatment based on their growth on MS medium was determined. Shoot tips of 82 clones after
irradiation with 0 (control treatment), 5, 10, 20, 30 and 40 Gy of Gamma ray were cultured on
MS medium for 6 weeks. The results showed that ward method on growth of in vitro shoots
from 82 clones at the fourth week resulted into 5 clusters. Cluster 3 was the best for growth of
A. annua shoot culture containing 14 clones e.i. 5.3.a , 5.4.a, 5.19.a1, 10.10.a*, 10.13.a, 10.37.a,
20.1.a, 20.6.a, 20.16.a 30.12.a, 30.15.a, 30.18.a, 30.28.a, 30.38.a. This cluster consisted of 3
clones came from irradiation 5 Gy, 3 clones from 10 Gy, 3 clones from 20 Gy, and 5 clones
from 30 Gy, respectively. The research finding was beneficial for selection of superior clones
for the genetic improvement.