INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 15, No. 03, Month JULY, Year 2019, Pages 399 - 408

The use of rapd marker for verification of dendrobium hybrid, d. santana x d. friedericksianum orchid

Choopeng, S. and Te-chato, S.

Abstract Download PDF

Dendrobium is one of the most important orchids due to its beautiful flower. The use of random amplified polymorphic DNA (RAPD) marker for verification of Dendrobium hybrid orchid between D. santana and D. friedericksianum was investigated. Twelve 10-mer primers of RAPD were used for the above purpose. The results showed that 6 primers which were OPA03, OPB07, OPB18, OPJ04, OPR11 and OPT06 could amplify and give a clear profiles of DNA from all samples. The primer OPA03240, 310, OPB07380, 420, 850, OPJ04550, 980, OPT06310, 450, 620, OPR11180, 500, 1,040, OPB18300, 410, 560, 1,010 could be used as markers for verification of hybrid between D. santana and D. friedericksianum.

Keywords

Orchid, Dendrobium hybrid, RAPD, primer

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