Virulence and genetics at the molecular level of an indigenous strain of beauveria bassiana affected by artificial and mass production media usage for sustainable insect control
Molecular biology of indigenous strain of entomopathogenic fungus, Beauveria
bassiana (Balsamo) Vuillenim (Ascomycota: Hypocreales) (strain Bbs01) based on DNA
fingerprinting and enzymatic studies and based on respective RAPD-PCR and acrylamide gel
electrophoresis were conducted. The results showed that neither artificial media (PDA, SDA,
SDAY, MEA, NA and WA) nor mass production material (cooked rice, paddy, millet and dog
feed) effect the fungal genetic stability and protease enzyme activity. Correlation analysis
indicated that pathogenicity of Bbs01 to cabbage aphids, Lipaphis erysimi (Hemiptera:
Aphididae) and flea beetle, Phyllotreta sinuata (Coleoptera: Chrysomelidae) were increased
with protease activity. Accordingly, the most suitable respective artificial and culture material
for B. bassiana Bbs01were SDAY and dog food.
Keywords
Beauveria bassiana, genetic stability, mass production media, sustainable insect control, virulence
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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