INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 15, No. 01, Month JANUARY, Year 2019, Pages 141 - 156

Production of exopolysaccharide from tricholoma crassum in submerged culture and its antioxidant activities

Thai, L. Q. and Keawsompong, S.

Abstract Download PDF

The effects of carbon, nitrogen sources and nutritional requirements were studied for the production of exopolysaccharide (EPS) from Tricholoma crassum using the statistically based experimental design in a shake flask culture, and the antioxidant activities of EPS in vitro. Glucose and Yeast extract was found to be the most suitable of carbon and nitrogen source. The optimization of submerged culture conditions was studied using Taguchi method; the results showed the optimized cultivation was pH 6.5, incubation temperature 30°C, cultivation time 10 days. Under these optimal conditions, the EPS content and dry cell weight were 0.727 g/L and 9.7 g/L, respectively. The EPS consisted of galactose, glucose and mannose with ratio 1:6.25:17.72, suggested that EPS from T. crassum could be Galacto-Gluco-Mannan. The EPS demonstrated positively antioxidant potential on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power and inhibition of lipid peroxidation assay.

Keywords

Tricholoma crassum, exopolysaccharide, Taguchi method, antioxidant

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