The cultivation of Antrodia cinnamomea on the different kinds of substrates was
investigated. Firstly, it was cultivated on sawdusts from five sources: Khae na (Dolichandrone
serrulata) , rain tree (Samanea saman) , mango (Mangifera indica), Para rubber tree (Hevea
brasiliensis) and cinnamon tree (Cinnamomum verum). It was found that the mushroom mycelia
grew well on mango sawdust: the average initial growth time was 9.40 days and the average time
the mycelia fully colonized the sawdust surface was 90.80 days. The mycelia had abundant
density and orange color at 70 days, with cinnamon odor at 110 days. Secondly, it was cultivated
on the barley (Hordeum vulgare), millet grass (Sorghum sp.), two varieties of rices (Oryza sativa):
Sang yod and Hom nin, black sesame (Sesamum indicum), job"es tears (Coix sp.) and mung bean
(Vigna radiata). It was found that the mycelia grew well on millet grass with initial growth time
in 9.86 days and it fully colonized the substrate in 26.14 days. The mycelia had abundant density
and orange color in 50 days, with cinnamon odor in 110 days. Finally, the mycelia were cultivated
on five liquid media: PD, PMD, PPD, PYD and PMPYD. It revealed that the mycelia grew well
on PMPYD. It had abundant density with yellow-orange colors when it was 90 days. The mycelia
fully covered the surface of the media in 60.00 days. Its colony was a thick mat and floated on the
surface of the liquid media. The average dry weight of the mycelia was 0.528 g/100 ml in 90 days
and the pH value of liquid media was increased during the growth of the mycelia. In addition,
when the quantities of the bioactive compounds, adenosine and cordycepin, from dried mycelia
which cultivated on the Mango sawdust, Millet grass seeds and the PMPYD were analyzed by the
HPLC, adenosine was found at 202.23, 25.25 and 81.90 mg/100 g dried weight mycelia,
respectively. However, 499.69 mg/100 g of cordycepin was found only in mycelia cultivated on
Mango sawdust, but not found in dried mycelia cultivated on Millet grass seeds and the PMPYD.
It revealed that the mycelia had different quantity nutrients such as protein, fat, carbohydrate, ash
and moisture.
Keywords
Antrodia cinnamomea, Cultivation of mushroom, Bioactive, Nutritional value
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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