In vitro antimicrobial activities of Carissa carandas L. fruit extracts dissolved in
water and their effect on biological qualities in ground pork during storage period for 8 days at
4 oC and at -20 oC for 12 weeks were investigated. The antimicrobial avctivity was carried out
using agar well diffusion method against food pathogenic and spoilage bacteria. For
antimicrobial testing, water extracts of C. carandas inhibited both pathogenic and spoilage
bacteria including Salmonella Typhimurium TISTR 292, Staphylococcus aureus TISTR 118,
Escherichia coli TISTR 780, Aeromonas hydrophila TISTR 1321, Pseudomonas fluorescens
TISTR 358 and Bacillus coagulans TISTR 1447, at the concentration of 50 mg/ml. The water
extract of C. carandas fruit was applied in ground pork. The ground pork samples were
subjected to four treatments including control (non-treated), 0.2 g BHT/kg meat, 2.5 g and 5 g
extracts /kg meat. Total microbial count, yeast/mold, psychrophilic bacteria and coliforms were
determined in ground pork during storage time for 0, 2, 4, 6 and 8 days at 4 oC and frozen
ground pork storage at -20 oC for 0, 4, 8, and 12 weeks. The results showed that pH was not
different in each treatments. Total plate count and coliforms bacteria was decreased after
adding 5 g extracts /kg meat at 6-8 days in chilled ground pork and up to 8 and 12 weeks in
frozen ground pork. This finding indicated that C. carandas fruit extracts could be used for
natural antimicrobial in ground pork for prolong shelf-life.
Keywords
Antimicrobial. Carissa carandas, extract
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
Published by : Association of Agricultural Technology in Southeast Asia (AATSEA) Contributions welcome at : http://www.ijat-aatsea.com
By using our website, you acknowledge that you have read and understand our Cookie Policy and Privacy Policy.