Water lettuce is a major problem in water resources and rapid widespread. The
management focused to change from herbicides to biological control using natural enemies of
water lettuce including fungal pathogens. The blighted and spotted leaf of diseased water
lettuce plants were collected from 8 provinces of Thailand. Thirty-nine isolates of fungal
pathogens were identified using morphological characteristics. They were identified as
Myrothecium 5 isolates, Chaetomium 2 isolates, Aspergillus 3 isolates, Rhizopus 3 isolates,
Nigrospora 6 isolates, Bipolaris 4 isolates, Curvularia 2 isolates, Epicoccum 5 isolates and
Acremonium 9 isolates. The fungal Myrothecium isolates, through the morphologically
identification process, were selected to study molecular data, which were derived from
sequencing the internal transcribed spacer (ITS) regions of the rDNA. The result indicated that
the five Myrothecium isolates could be identified as M. inundatum. Five isolates of M.
inundatum were tested pathogenicity for controlling water lettuce. Fungal isolates were found
to be the virulent pathogenicity on water lettuce.
Keywords
biocontrol, water lettuce, fungal pathogens
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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