Yardlong bean is an important legume of Southeast and East Asia. It is believed to
have been domesticated from vegetable (pod) cowpea. Among domestication-related traits, seed
size is a distinctly trait that distinguish yardlong bean from its wild ancestor which has resulted
in an approximately three-fold increase in seed length. Previously, we identified major QTLs
for seed-related traits on linkage group 7, which were located on pleiotropic quantitative loci.
Seed-related traits are highly complex quantitative traits that are controlled by multiple
quantitative loci (QTLs) with a major and several minor effects and are influenced by multiple
genetic and environmental factors. Thus, it is challenging to identify the major genes for
controlling seed-related traits in yardlong bean. As the basis for fine mapping, a set of near
isogenic lines (NILs) was developed from the cross between yardlong bean (JP81610) and wild
cowpea (JP89083) population based on three generations of backcrossing and three generations
of selfing. We have been able to narrow down the location of the genes underlying seed-related
traits from 4.3 Mbp to 1.65 Mbp region. The locus was associated with transgressive variation
for seed-and pod-related traits in this population. The phenotype was difficult to evaluate due to
the influence of pod-related traits (pod length, pod width and pod softness) affected to seed size
variation, underscoring the value of using multiple approaches to phenotyping, including
extreme sampling and NILs group-mean comparisons. The fact that the QTLs controlling podrelated
traits have also been detected on this target region, in which the genes for seed-related
traits were associated, suggest that this region may generally not randomly distributed across
the genome.
Keywords
yardlong bean, QTL, fine mapping
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
Published by : Association of Agricultural Technology in Southeast Asia (AATSEA) Contributions welcome at : http://www.ijat-aatsea.com
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