Efficacy of herbal extracts to control multi-antibiotics resistant (mar) aeromonas veronii isolated from motile aeromonas septicemia (mas)-exhibiting nile tilapia (oreochromis niloticus)
U-taynapun, K., Mueangkan, N. and Chirapongsatonku, N.
Motile Aeromonas septicemia (MAS) is a disease causing a devastating loss in fish
farming including Nile tilapia (Oreochromis niloticus). The disease is caused by pathogenic
bacteria Aeromonas spp. Our previous study has revealed that A. verronii is one of commonly
isolated species from the MAS–expressing Nile tilapia cultured in Southern Thailand. Among
the collected bacteria, one isolate exhibiting multi-antibiotic resistance (MAR) against
tetracycline and oxyteracycline was selected as a model for testing the biological control ability
of herbal extracts. The herbal extracts obtained from 6 herbal plants; Caesalpinia sappan (Cs),
Allium sativum (As), Illicium verum (Iv), Alpinia galanga (Ag), Piper longum (Pl), and
Foeniculum vulgare (Fv), extracted using 3 different solvents; 95% ethanol (E95), water (W)
and soybean oil (O) were first screened for their inhibitory potential by disc diffusion method
compared with tetracycline (30 μg). The clear zones (7–18 mm) were detected in Cs-E95, Cs-
W, As-E95, Iv-E95, IV-W, and Ag-E95 while the clear zone in tetracycline was about 13
mm. All 6 herbal extracts showing clear zone were further evaluated for their minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against the
MAR–A. veronii. The greatest MIC of 0.469 mg/ml was recorded in Cs-E95 and Cs-W and
MBC of 0.938 mg/ml in Cs-W. Moreover, the inhibitory effect of Cs-E95, at the concentration
of MIC and MBC, on the growth of MAR–A. veronii cultured under the water temperature
observed in the fish farm was also demonstrated. This finding revealed the potential of herbal
extracts as a favorably health and environmental friendly approach in modern aquaculture to
control the bacterial pathogens instead of using antibiotics.