INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 14, No. 07, Month DECEMBER, Year 2018, Pages 2171 - 2180

The effect of plant growth regulator and in vitro conservation of teak (tectona grandis l.) by tissue culture

Tongsad, P., Laipasu, P., Chareonsap, P. P. and Poeaim, A.

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The plant regeneration of teak (Tectona grandis L.) was reported. Result showed that the highest number of shoot induction was 55.49 cm., in length of shoots which tested at 0.25 mg·L-1 BAP, and averaged 40.29 cm which tested at 0.25 mg·L-1 mT after 4 weeks. The shoots were elongated using shoot tip explants of cryopreservation on teak by encapsulation-dehydration (EnDe), encapsulation-vitrification (EnVi) and vitrification (Vi) techniques. The shoot tips were used as explants, encapsulated apices were precultured on hormone-free MS medium containing 0.3 M sucrose for 2 days, dehydrated, and immersed in liquid nitrogen for 24 h after that thawed in thermal bath at 40 ºC and washed in unloading solution. Vi and EnVi protocols were used to treat with a loading solution, dehydrated by plant vitrification solution 2 (PVS2). EnDe protocol were encapsulated by desiccated air-dry in laminar air flow and dehydrated with silica gel. Results showed that Vi gave an excellent shoot tip explants for survival and regrowth. Vi were unable to survival. This experiment was preliminary searched to improve and develop the teak for cryopreservation.

Keywords

Cryopreservation, Encapsulation-dehydration, Encapsulation-vitrification, Plant regeneration, Vitrification

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