Thunbergia laurifolia Lindl. leaves were extracted by various ratios (0, 25, 50, 75 and
100%) of ethanol in water as solvents for crude extraction. The highest crude extract yield was
obtained by using 25% ethanol solvent with three time extractions. The inhibitory effect of each
extract on seed germination and seedling growth of Amaranthus gracilis was investigated. The
results indicated that the 100% ethanol extract expressed the best inhibitory effect. At
concentration of 1250 ppm, the 100% ethanol extract completely inhibited seed germination of
A. gracilis. The 100% ethanol extract also inhibited α-amylase activities in A. gracilis seed
during seed germination. However, the extract does not show effect on seed imbibition. The
inhibition of induction of α-amylase increased with increasing concentration of the 100%
ethanol extract.
Keywords
Thubergia laurifolia, Allelopathy, Solvent, Weed control
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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