INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 14, No. 07, Month DECEMBER, Year 2018, Pages 1969 - 1976

Possibility of split mushroom schizophyllum commune byproduct extracts as antimicrobial and antioxidant agent for aquaculture

Srichanun, M, Lerssutthichawal, T., Nganwisuthiphan, T. and Chirapongsatonkul, N.

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Schizophyllum commune by product (SCB) extracted was investigated from different organic solvent having the different relative polarity consisting of hexane, ethyl acetate, dichloromethane, 2-butanol, ethanol, methanol and water as antimicrobial and antioxidant agent in order to added value of waste from agriculture. The antibacterial assay to pathogenic bacteria for economic fish culture Aeromonas hydrophila and Streptococcus agalactiae were tested by disc diffusion method. Antioxidant capacity was measured by DPPH assays. The result found that the inhibitory to pathogenic bacteria was not observed in all SCB extract compared to positive disc of commercial antibiotic (oxytetracyclin and norfloxacin) at the concentration of 20,000 ppm. While, the DPPH radical scavenging activity of each extract was significantly different (p <0.05). SCB extracted by methanol showed the highest activity (IC50=23 g/mL) with no significant difference to SCB extracted by 2-butanol. The lowest level was observed in SCB extracted by ethanol and ethyl acetate (IC50=35 ug/mL). This study indicated that the different of organic solvent gave the different capacity of antioxidant level and SCB extract might be used as antioxidant agent in aquaculture.

Keywords

Schizophyllum commune, Mushroom by-product, antioxidant, antimicrobial

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