Musty odor due to geosmin and 2-methylisoborneol (MIB) is currently the most
serious economic problem faced by aquaculture export industry. They are susceptible to
biodegradation with many microorganisms responsible for their removal from water. The
study reported the potential microorganism which isolated from 10 Tilapia farming in
northern Thailand on the degradation of musty odor. Microbial isolation was done using the
media containing geosmin and MIB standard solution. Twenty isolates were selected in
order to test their efficiencies on degradation of musty odor. After 48 h. of incubation,
isolate TS1 was most efficient to reduce geosmin in the media (92.16%) as analyzed by
headspace solid phase micro-extraction (HS-SPME) and gas chromatography–mass
spectrometry (GC/MS). Whereas, isolate LS6 had optimum efficiency to reduce MIB
(76.61%). Five species (Achromobacter denitrificans, Delftia tsuruhatensis, Comamonas
sp., Acinetobacter calcoaceticus and Raoultella ornithinolytica) with optimal efficiency to
reduce geosmin, and MIB were then in vivo tested for pathogenesis in fishes. It was shown
that all the bacterial species did not cause disease in the fish with survival rate of 96.67-
100%. After 48 h. of incubation, Comamonas sp. and Ach. denitrificans had the highest
efficiency to reduce geosmin and MIB (about 40% removal). Microbial cultures in the
water with subsequent community profile analysis using 16S rRNA-directed PCR-DGGE
identified uncultured bacterial species were grown after 48 h. of incubation. Five bacteria
most likely involved in the biodegradation of musty odor within the pond water.