The result was explained an effect of Plant Growth Regulators (PGRs) for young
seeds induction and shoot multiplication of Gluta usitata (Na-pong3) by plant tissue culture
technique to preserve and increased plant propagation. The young seeds were induced by
comparison to 0.5, 1, 1.5, 2 and 3 mg/L 6-Benzyl amino purine (BAP), Thidiazuron (TDZ) or
meta-Topolin (mT) on Woody Plant Medium (WPM). The results showed the highest
percentage of seed regeneration was 1.5 mg/L BAP which was100% germination. The shoots
was highest induced by 0.5 mg/L BAP after culture for 6 weeks. The shoots were transferred to
WPM medium containing with BAP, TDZ or mT for multiple shoot induction. The maximum
number of shoots were observed on WPM medium combined with 1.5 mg/L BAP (about 6
shoots per explant) after subcultured for three to four times. The best of root induction was
observed on WPM medium without PGRs.
Keywords
Gluta usitata, shoot multiplication, young seeds regeneration
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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