The antagonistic potential of Neosartorya and Talaromyces proved to control leaf
spot of kale caused by Alternaria brassicicola. Leaf spot symptoms were collected from
Nakornpathom, Suphanburi, Ratchaburi and Nonthaburi provinces, isolated and identified the
pthogen by morphological and molecular characterization. Four isolates were confirmed as A.
brassicicola. The pathogenicity test was done by detached leaf bioassay and found that AbKNP01
was the highest virulence pathogenic isolate. Dual culture technique was used to assay
with eight species of antagonistic fungi (N. hiratsukae, N. pseudofischeri, N. aureola, N.
spinosa, N. fennelliae, Neosartorya sp., T. trachyspermus and T. muroii) against A.
brassicicola. The best effective of antagonist to control the tested pathogen was N. spinosa
CHA09-A01.