The influence of dietary energy level on the degradation of troponin-T protein
and expression of calpain and calpastatin genes in native Thai cattle was determined.
Eighteen steers (at 200 days old and average body weight of 100 ± 20 kg) were randomly
assigned to 3 dietary treatment groups of different metabolizable energy: Treatment 1 [(8.9
MJ/ kg DM; n=6), Treatment 2 (9.7 MJ/kg DM; n=6) and Treatment 3 (10.5 MJ/kg DM;
n=6)]. The diets were in the form of fermented total mixed ration (FTMR). At the end of
520 days of feeding, the steers attained 300 ± 10 kg BW at slaughter. The Longissimus
dorsi m. samples collected within 2 d postmortem were further aged for 14 d for the
troponin-T degradation immunoblot analysis while, the CAPN1, CAPN2, and CAST genes
expression analyses only used samples at 2 d postmortem. The relative bands intensities of
intact (37 kDa) and degraded troponin-T (30 kDa) were unaffected by the dietary
treatments (p>0.05). Higher 37 kDa (p<0.01) and 30 kDa (p<0.001) troponin-T proteins
expression were noted at 2 d and 14 d post-mortem, respectively. The CAPN1, CAPN2,
and CAST genes expression remained unaffected (p>0.05) by the dietary treatment.
Nonetheless, significant associations were observed between CAPN2 and CAST genes
expression (r=0.93; p<0.0001), and between CAPN1 gene expression and 30 kDa troponin-
T proteins expression at 14 d postmortem (r=0.58; p<0.05). This study concluded that
neither troponin-T proteins nor calpain/ calpastatin genes expression were influenced by
dietary energy levels in fattened native Thai cattle. The associations between CAPN2 and
CAST genes expression, and between CAPN1 gene expression and 30 kDa troponin-T
proteins expression at 14 d postmortem suggest the involvement of CAPN1 in myofibrillar
proteolysis during the 14 d postmortem ageing of Longissimus dorsi m. in native Thai cattle.